ITGA5 antibody

Catalog No. ABIN2689316

This Rat Monoclonal antibody specifically detects ITGA5 in FACS, IHC (fro), IF, IHC (p) and BR. It exhibits reactivity toward Mouse and has been mentioned in 10+ publications.

Quick Overview for ITGA5 antibody (ABIN2689316)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR)

Clone: 5H10

Product Details anti-ITGA5 Antibody

Brand: BD Pharmingen™

Characteristics: The 5H10-27 (MFR5) antibody reacts with the α5 chain of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, mast cells, and a variety of mouse cell lines, but not splenocytes. Soluble 5H10-27 (MFR5) antibody has been reported to inhibit VLA-5-mediated functions in vitro. In addition, immobilized mAb 5H10-27 (MFR5) has been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3e antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD49e - Purified - Clone 5H10-27 (MFR5) - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse mast cell line MC/9

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ITGA5 antibody (ABIN2689316)

Makwana, Werner, Acosta-Saltos, Gonitel, Pararajasingam, Pararajasingham, Ruff, Rumajogee, Cuthill, Galiano, Bohatschek, Wallace, Anderson, Mayer, Behrens, Raivich: "Peripheral facial nerve axotomy in mice causes sprouting of motor axons into perineuronal central white matter: time course and molecular characterization." in: The Journal of comparative neurology, Vol. 518, Issue 5, pp. 699-721, (2010) (PubMed).

Cukierman, Pankov, Stevens, Yamada: "Taking cell-matrix adhesions to the third dimension." in: Science (New York, N.Y.), Vol. 294, Issue 5547, pp. 1708-12, (2001) (PubMed).

Yang, Hynes: "Fibronectin receptor functions in embryonic cells deficient in alpha 5 beta 1 integrin can be replaced by alpha V integrins." in: Molecular biology of the cell, Vol. 7, Issue 11, pp. 1737-48, (1997) (PubMed).

Rich, Van Nood, Lee: "Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2916-23, (1996) (PubMed).

Ruppert, Aigner, Hubbe, Yagita, Altevogt: "The L1 adhesion molecule is a cellular ligand for VLA-5." in: The Journal of cell biology, Vol. 131, Issue 6 Pt 2, pp. 1881-91, (1996) (PubMed).

Uhlenkott, Huijzer, Cardeiro, Elstad, Meadows: "Attachment, invasion, chemotaxis, and proteinase expression of B16-BL6 melanoma cells exhibiting a low metastatic phenotype after exposure to dietary restriction of tyrosine and phenylalanine." in: Clinical & experimental metastasis, Vol. 14, Issue 2, pp. 125-37, (1996) (PubMed).

Gomez, Cano: "Expression of beta 1 integrin receptors in transformed mouse epidermal keratinocytes: upregulation of alpha 5 beta 1 in spindle carcinoma cells." in: Molecular carcinogenesis, Vol. 12, Issue 3, pp. 153-65, (1995) (PubMed).

Halvorson, Coligan: "Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4567-74, (1995) (PubMed).

Hardy, Minguell: "Modulation of the adhesion of hemopoietic progenitor cells to the RGD site of fibronectin by interleukin 3." in: Journal of cellular physiology, Vol. 164, Issue 2, pp. 315-23, (1995) (PubMed).

Kinashi, Springer: "Adhesion molecules in hematopoietic cells." in: Blood cells, Vol. 20, Issue 1, pp. 25-44, (1995) (PubMed).

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: CD49e

Background: Synonyms: Integrin α5 chain

Pathways: Integrin Complex