CD6 antibody
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- Target See all CD6 Antibodies
- CD6
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD6 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Brand
- BD Pharmingen™
- Characteristics
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The OX-52 antibody reacts with CD6, a 95-120- kDa antigen on T lymphocytes and thymocytes expressing αβ and γδ Tcell receptors. Rat NK cells express low levels of CD6 and are CD8a+, CD8b-, CD5-, and CD3-. In immunoprecipitation experiments, CD6 has been shown to co-precipitate with CD5 and to play a key role in CD5 tyrosine phosphorylation. Two-color evaluation of splenic T lymphocytes. LEW splenocytes were simultaneously stained with PE-conjugated anti-rat CD3 mAb G4.18 (Cat. No. 554833) and purified mAb OX-52 (right panel), followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 (Cat. No. 553390). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ Purified Mouse Anti-Rat CD6 - Purified - Clone OX-52 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.1 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- PVG-RT1c rat thoracic-duct leukocytes and splenocytes
- Clone
- OX
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product CD6 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C.
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OX52 is the rat homologue of CD6: evidence for an effector function in the regulation of CD5 phosphorylation." in: Journal of leukocyte biology, Vol. 73, Issue 1, pp. 183-90, (2003) (PubMed).
: "Identification and characterization of rat T cell subpopulations expressing T cell receptors alpha/beta and gamma/delta." in: European journal of immunology, Vol. 20, Issue 2, pp. 343-9, (1990) (PubMed).
: "MRC OX-52: a rat T-cell antigen." in: Immunology, Vol. 57, Issue 4, pp. 527-31, (1986) (PubMed).
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OX52 is the rat homologue of CD6: evidence for an effector function in the regulation of CD5 phosphorylation." in: Journal of leukocyte biology, Vol. 73, Issue 1, pp. 183-90, (2003) (PubMed).
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- Target
- CD6
- Alternative Name
- CD6 (CD6 Products)
- Synonyms
- TP120 antibody, OX52 antibody, CD6 antigen antibody, CD6 molecule antibody, Cd6 molecule antibody, Cd6 antibody, CD6 antibody
- Background
- Synonyms: OX-52 Antigen, Pan-T Cells
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