Integrin beta 3 antibody

Catalog No. ABIN2689352

This Armenian Hamster Monoclonal antibody specifically detects Integrin beta 3 in FACS, IHC (p), IHC (fro) and BR. It exhibits reactivity toward Mouse and Rat. It has been mentioned in 7+ publications

Quick Overview for Integrin beta 3 antibody (ABIN2689352)

Target: Integrin beta 3 (ITGB3)

Reactivity: Mouse, Rat

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Clone: 2C9-G2

Product Details anti-Integrin beta 3 Antibody

Characteristics: The 2C9.G2 antibody reacts with the integrin β3 chain (CD61), which associates with the integrin αv chain (CD51) to form the vitronectin receptor and with the αIIb chain (CD41) to form the gpIIb/IIIa complex. Both receptors mediate adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is also mediated by the binding of αvβ3 integrin or vitronectin receptor to CD31 (PECAM-1). In addition, interaction of the αvβ3 integrin with its ligands regulates the L-type Ca2+ channel in vascular smooth muscle cells, possibly mediating vasodilatory responses to injury. Soluble and insoluble 2C9.G2 mAb mimics the effect of the natural ligands in smooth muscle cells from rat cremaster arterioles. Furthermore, osteopontin, also named Eta-1, is a cytokine that binds to αvβ3. CD61 is expressed on platelets, activated T lymphocytes, polymorphonuclear granulocytes, and blastocysts. Cross-reactivity of mAb 2C9.G2 to rat mast cells and platelets has been observed by flow cytometric analysis. mAb 2C9.G2 has been demonstrated to block binding of rat and mouse cells to fibronectin.

BD Pharmingen™ Purified Hamster Anti-Mouse CD61 - Purified - Clone 2C9.G2 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms, Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse T-cell Hybridoma 2B4 Vitronectin Receptor

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Integrin beta 3 antibody (ABIN2689352)

Ashkar, Weber, Panoutsakopoulou, Sanchirico, Jansson, Zawaideh, Rittling, Denhardt, Glimcher, Cantor: "Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity." in: Science (New York, N.Y.), Vol. 287, Issue 5454, pp. 860-4, (2000) (PubMed).

Wu, Mogford, Platts, Davis, Meininger, Davis: "Modulation of calcium current in arteriolar smooth muscle by alphav beta3 and alpha5 beta1 integrin ligands." in: The Journal of cell biology, Vol. 143, Issue 1, pp. 241-52, (1998) (PubMed).

Frieser, Hallmann, Johansson, Vestweber, Goodman, Sorokin: "Mouse polymorphonuclear granulocyte binding to extracellular matrix molecules involves beta 1 integrins." in: European journal of immunology, Vol. 26, Issue 12, pp. 3127-36, (1997) (PubMed).

Piali, Hammel, Uherek, Bachmann, Gisler, Dunon, Imhof: "CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 451-60, (1995) (PubMed).

Yasuda, Hasunuma, Adachi, Sekine, Sakanishi, Hashimoto, Ra, Yagita, Okumura: "Expression and function of fibronectin binding integrins on rat mast cells." in: International immunology, Vol. 7, Issue 2, pp. 251-8, (1995) (PubMed).

Kieffer, Phillips: "Platelet membrane glycoproteins: functions in cellular interactions." in: Annual review of cell biology, Vol. 6, pp. 329-57, (1991) (PubMed).

Moulder, Roberts, Shevach, Coligan: "The mouse vitronectin receptor is a T cell activation antigen." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 343-7, (1991) (PubMed).

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: CD61

Background: Synonyms: Integrin β3 chain

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex