The HM79b antibody reacts with an extracellular epitope of Ig β chain (Igβ or CD79b), a 35-40- kDa transmembrane protein which forms an 80-90- kDa disulfide-linked heterodimer with Ig α chain (Igα or CD79a, 30-35 kDa). On mature B lymphocytes, the CD79a/CD79b heterodimers are non-covalently associated with surface IgM to form the B-cell receptor complex (BCR). The presence of CD79a/CD79b is necessary for surface expression of the BCR and signal transduction via the BCR in B lymphocytes and pre-B cells. It was recently reported that CD79b may be expressed on the cell surface preceding the appearance of surface IgM during B-lymphocyte development. At this pro-B-cell stage, CD79b participates in signal transduction involved in the regulation of B-cell development. It should be noted that multi-parameter flow cytometric analyses of bone marrow suspensions performed have been unable to detect surface staining by HM79b mAb on CD45R/B220+ IgM- cells. Two-color analysis of the expression of CD79b on mouse bone marrow cells. A single-cell suspension of BALB/c bone marrow was simultaneously stained with FITC-conjugated anti-mouse CD45R/B220 RA3-6B2 (Cat. No. 553087/553088) and purified HM79b monoclonal antibodies, followed by PE-conjugated anti-hamster IgG cocktail (Cat. No. 554056). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Purified Hamster Anti-Mouse CD79b - Purified - Clone HM79b (also known as HM79-16) - Isotype Armenian Hamster IgG2, λ1 - Reactivity Ms - 0.5 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
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