-
- Target See all CD80 Antibodies
- CD80
-
Reactivity
- Rat
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This CD80 antibody is conjugated to Biotin
-
Application
- Flow Cytometry (FACS)
- Brand
- BD Pharmingen™
- Characteristics
-
The 3H5 antibody reacts with CD80 (B7-1), a member of the Ig superfamily of transmembrane proteins. CD80, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. CD80 is predominantly expressed on antigen-presenting cells. It can be induced on splenic B cells by in vitro polyclonal activation such as LPS treatment. 3H5 mAb is reported to block the costimulatory function of rat CD80 and to immunoprecipitate CD80 from Lewis-S1 cell lysates. CD80 expression on spleen cells. 72-hour LPS-stimulated LOU splenocytes were simultaneously stained with FITC-conjugated anti-rat CD45RA mAb OX-33 (Cat. no. 554883) and biotinylated mAb 3H5, followed by Streptavidin-PE (Cat. no. 554061). Viable lymphocyte blasts are represented. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ Biotin Mouse Anti-Rat CD80 - Biotin - Clone 3H5 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.5 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Rat HTLV-1-transformed T-cell line Lewis-S1
- Clone
- 3H5
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD80 Primary Antibody
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
-
-
Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." in: Journal of leukocyte biology, Vol. 64, Issue 6, pp. 803-9, (1998) (PubMed).
: "Characterization of rat CD80 and CD86 by molecular cloning and mAb." in: International immunology, Vol. 9, Issue 7, pp. 993-1000, (1997) (PubMed).
: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).
: "
-
Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." in: Journal of leukocyte biology, Vol. 64, Issue 6, pp. 803-9, (1998) (PubMed).
-
- Target
- CD80
- Alternative Name
- CD80 (CD80 Products)
- Synonyms
- B7 antibody, B7-1 antibody, B7.1 antibody, BB1 antibody, CD28LG antibody, CD28LG1 antibody, LAB7 antibody, B71 antibody, Cd28l antibody, Ly-53 antibody, Ly53 antibody, MIC17 antibody, TSA1 antibody, CD80 molecule antibody, Cd80 molecule antibody, CD80 antigen antibody, CD80 antibody, Cd80 antibody
- Background
- Synonyms: B7-1
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
-