Disialoganglioside 2 (GD2) antibody

Catalog No. ABIN2689490

This Mouse Monoclonal antibody specifically detects Disialoganglioside 2 in FACS, CyTox, IHC (fro) and IF. It exhibits reactivity toward Human and Mouse. It has been mentioned in 5+ publications

Quick Overview for Disialoganglioside 2 (GD2) antibody (ABIN2689490)

Target: Disialoganglioside 2 (GD2)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Clone: 14-G2a

Product Details

Characteristics: Gangliosides are sialic-acid bearing glycolipids that are expressed on the surface of all mammalian cells, and are likely involved in mediating cell-substratum interactions. They are important target antigens for antibody dependent cellular cytotoxicity (ADCC) of human melanoma and neuroblastoma cells. Human melanoma cells produce gangliosides, designated as GD2 and GD3 which are deposited in the subtratum-attached material, and may play a significant role in the melanoma metastatic phenotype.

BD Pharmingen™ Purified Mouse Anti-Human Disialoganglioside GD2 - Purified - Clone 14.G2a - Isotype Mouse IgG2a - Reactivity Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: LAN-1 human neuroblastoma cells

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Frost, Hank, Reaman, Frierdich, Seeger, Gan, Anderson, Ettinger, Cairo, Blazar, Krailo, Matthay, Reisfeld, Sondel: "A phase I/IB trial of murine monoclonal anti-GD2 antibody 14.G2a plus interleukin-2 in children with refractory neuroblastoma: a report of the Children's Cancer Group." in: Cancer, Vol. 80, Issue 2, pp. 317-33, (1997) (PubMed).

Cheresh, Pierschbacher, Herzig, Mujoo: "Disialogangliosides GD2 and GD3 are involved in the attachment of human melanoma and neuroblastoma cells to extracellular matrix proteins." in: The Journal of cell biology, Vol. 102, Issue 3, pp. 688-96, (1986) (PubMed).

Cheresh, Rosenberg, Mujoo, Hirschowitz, Reisfeld: "Biosynthesis and expression of the disialoganglioside GD2, a relevant target antigen on small cell lung carcinoma for monoclonal antibody-mediated cytolysis." in: Cancer research, Vol. 46, Issue 10, pp. 5112-8, (1986) (PubMed).

Cheresh, Honsik, Staffileno, Jung, Reisfeld: "Disialoganglioside GD3 on human melanoma serves as a relevant target antigen for monoclonal antibody-mediated tumor cytolysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 15, pp. 5155-9, (1985) (PubMed).

Hakomori: "Tumor-associated carbohydrate antigens." in: Annual review of immunology, Vol. 2, pp. 103-26, (1985) (PubMed).

Target Details

Target: Disialoganglioside 2 (GD2)

Alternative Name: Disialoganglioside GD2

Background: Synonyms: GD2