Galectin 3 antibody

Catalog No. ABIN2689513

Cited in 4+ publications. The Mouse Monoclonal anti-Galectin 3 antibody (Clone B2C10) (ABIN2689513) specifically detects Galectin 3 in IHC (f) and ICS. The antibody is reactive with Human samples.

Quick Overview for Galectin 3 antibody (ABIN2689513)

Target: Galectin 3 (LGALS3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Galectin 3 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS)

Clone: B2C10

Product Details anti-Galectin 3 Antibody

Characteristics: Reacts with Galectin-3, a 30 kDa protein, a member of a newly defined family of β-galactoside-binding animal lectins. It has been previously referred to as IgE-binding protein (εBP) and carbohydrate-binding protein 35 (CBP35). It is expressed on many tissues and cell types. Galectin-3 can be found in the nucleus and/or cytoplasm, on the cell surface and in the extra cellular matrix. Its function has been found to be diverse. It has been reported to be markedly elevated in the nucleus of proliferating cells, suggesting that Galectin-3 may play a role in cell growth regulation. Studies of Galectin-3 on the cell surface have shown it to be a non-integrin, laminin-binding protein, suggesting its role in cell adhesion to basement membranes. Monoclonal antibody B2C10 is suitable for immunohistochemical staining of parafin-embedded tissue sections. Profile of permeabilized peripheral blood monocytes analyzed by flow cytometry. Second step staining with Cat. No. 555988.

BD Pharmingen™ Purified Mouse Anti-Human Galectin-3 - Purified - Clone B2C10 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Galectin 3 antibody (ABIN2689513)

Cortegano, del Pozo, Cárdaba, de Andrés, Gallardo, del Amo, Arrieta, Jurado, Palomino, Liu, Lahoz: "Galectin-3 down-regulates IL-5 gene expression on different cell types." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 1, pp. 385-9, (1998) (PubMed).

Liu, Hsu, Zuberi, Hill, Shenhav, Kuwabara, Chen: "Modulation of functional properties of galectin-3 by monoclonal antibodies binding to the non-lectin domains." in: Biochemistry, Vol. 35, Issue 19, pp. 6073-9, (1996) (PubMed).

Mey, Leffler, Hmama, Normier, Revillard: "The animal lectin galectin-3 interacts with bacterial lipopolysaccharides via two independent sites." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 4, pp. 1572-7, (1996) (PubMed).

Cherayil, Weiner, Pillai: "The Mac-2 antigen is a galactose-specific lectin that binds IgE." in: The Journal of experimental medicine, Vol. 170, Issue 6, pp. 1959-72, (1990) (PubMed).

Target Details for Galectin 3

Target: Galectin 3 (LGALS3)

Alternative Name: Galectin-3

Target Type: Chemical

Pathways: RTK Signaling