GM-CSF antibody

Catalog No. ABIN2689524

There are 4+ publications for this product available. The Rat Monoclonal anti-GM-CSF antibody is suitable to detect GM-CSF in samples from Mouse. It has been validated for WB, ELISA, Neut and Inhibition.

Quick Overview for GM-CSF antibody (ABIN2689524)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut), Blocking Antibody (Inhibition)

Clone: MP1

Product Details anti-GM-CSF Antibody

Characteristics: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage-colony stimulating factor (GM-CSF). The immunogen used to generate the MP1-22E9 hybridoma was yeast-expressed recombinant mouse GM-CSF. This is a neutralizing antibody.

BD Pharmingen™ Purified Rat Anti-Mouse GM-CSF - Purified - Clone MP1-22E9 - Isotype Rat IgG2a - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant mouse GM-CSF

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-GM-CSF antibody (ABIN2689524)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Nozaki, Abrams, Pearce, Sauder: "Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin 1 in Pam 212 keratinocytes." in: The Journal of investigative dermatology, Vol. 97, Issue 1, pp. 10-4, (1991) (PubMed).

Suda, OGarra, MacNeil, Fischer, Bond, Zlotnik: "Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas." in: Cellular immunology, Vol. 129, Issue 1, pp. 228-40, (1990) (PubMed).

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin