IL-10 antibody (Biotin)

Catalog No. ABIN2689726

Product Details anti-IL-10 Antibody (Biotin)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Human, Virus

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-10 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Brand: BD Pharmingen™

Characteristics: The JES3-12G8 antibody reacts with human interleukin-10 (IL-10) and viral IL-10. The immunogen used to generate the JES3-12G8 hybridoma was human IL-10. This is a neutralizing antibody. This antibody is routinely tested by ELISA detection. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Anti-Human and Viral IL-10 - Biotin - Clone JES3-12G8 - Isotype Rat IgG2a - Reactivity Hu, Vir - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human IL-10

Clone: JES3

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IL-10 antibody (Biotin) (ABIN2689726)

Burdin, Péronne, Banchereau, Rousset: "Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10." in: The Journal of experimental medicine, Vol. 177, Issue 2, pp. 295-304, (1993) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Gotlieb, Abrams, Watson, Velu, Berek, Martínez-Maza: "Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers." in: Cytokine, Vol. 4, Issue 5, pp. 385-90, (1992) (PubMed).

Yssel, De Waal Malefyt, Roncarolo, Abrams, Lahesmaa, Spits, de Vries: "IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2378-84, (1992) (PubMed).

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10 (IL10 Products)

Synonyms: CSIF antibody, GVHDS antibody, IL-10 antibody, IL10A antibody, TGIF antibody, Il-10 antibody, IL10X antibody, interleukin 10 antibody, IL10 antibody, Il10 antibody

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints