IL-2 antibody

Catalog No. ABIN2689749

The Rat Monoclonal anti-IL-2 antibody has been validated for WB, ELISA, IP, FACS and Neut. It is suitable to detect IL-2 in samples from Mouse. There are 4+ publications available.

Quick Overview for IL-2 antibody (ABIN2689749)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Neutralization (Neut)

Clone: JES6

Product Details anti-IL-2 Antibody

Brand: BD Pharmingen™

Characteristics: The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2). The immunogen used to generate the JES6-1A12 hybridoma was recombinant mouse IL-2. This is a neutralizing antibody. This antibody is routinely tested by ELISA Capture. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse IL-2 - Purified - Clone JES6-1A12 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Mouse IL-2

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL-2 antibody (ABIN2689749)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Mo, Sarawar, Doherty: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, (1995) (PubMed).

Sarawar, Sangster, Coffman, Doherty: "Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1246-53, (1994) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation