IL-2 antibody

Catalog No. ABIN2689751

Product Details anti-IL-2 Antibody

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: ELISA, Blocking Antibody (Inhibition)

Brand: BD Pharmingen™

Characteristics: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2). The immunogen used to generate the MQ1-17H12 hybridoma was recombinant human IL-2. Unconjugated or purified forms of this antibody have been reported to be neutralizing for human IL-2 bioactivity. Expression of IL-2 by stimulated CD3+ human PBMC. Human PBMCs were stimulated for 6 hours with PMA (50 ng/mL final concentration, Sigma, Cat. #P-8139) and calcium ionophore A23187 (500-100 ng/mL final concentration, Sigma, Cat. #C-9275) in the presence of BD GolgiStop™ (2 μM final concentration, Cat. No. 554724). The PBMCs were stained with PE-Cy™5-anti-CD3 (PE-Cy™5-UCHT1, Cat. No. 555334), fixed, permeabilized, and then stained with 0.25 μg of FITC-rat anti-human IL-2 antibody (FITC-MQ1-17H12, Cat. No. 554565) using Pharmingen's staining protocol (left panel). To demonstrate specificity of staining, the binding of FITC-MQ1-17H12 was blocked by the preincubation of the fluorochrome - conjugated antibody with recombinant human IL-2 (10 ng/mL final concentration, Cat. No. 554603, middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled MQ1-17H12 antibody (5.0 μg, Cat. No. 554563, right panel) prior to staining with the FITC-MQ1-17H12 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking (middle panel) and unlabeled antibody blocking (right panel) specificity controls.

BD Pharmingen™ Purified Rat Anti-Human IL-2 - Purified - Clone MQ1-17H12 - Isotype Rat IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human IL-2 Recombinant Protein

Clone: MQ1

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL-2 antibody (ABIN2689751)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Andersson, Abrams, Björk, Funa, Litton, Agren, Andersson: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, (1995) (PubMed).

Fernandez, Andersson, Andersson, Troye-Blomberg: "Cytokine synthesis analyzed at the single-cell level before and after revaccination with tetanus toxoid." in: European journal of immunology, Vol. 24, Issue 8, pp. 1808-15, (1994) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2 (IL2 Products)

Synonyms: IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation