IL-2 antibody

Catalog No. ABIN2689752

The Rat Monoclonal anti-IL-2 antibody has been validated for ELISA, IP and Inhibition. It is suitable to detect IL-2 in samples from Mouse. There are 3+ publications available.

Quick Overview for IL-2 antibody (ABIN2689752)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Blocking Antibody (Inhibition)

Clone: JES6

Product Details anti-IL-2 Antibody

Brand: BD Pharmingen™

Characteristics: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2) and has been reported to be neutralizing. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/c mice were stimulated for 5 hours with hamster anti-mouse CD3 (2 μg/mL final concentration, clone 145-2C11, Cat. No. 553057) and hamster anti-mouse CD28 (2 μg/mL final concentration, clone 37.51, Cat. No. 553294) antibodies in the presence of BD GolgiStop™ (3 μM final concentration, Cat. No. 554724). The cells were harvested, stained with 0.06 μg of PE-conjugated rat anti-mouse CD4 (PE-RM4-5, Cat. No. 553049), fixed, permeabilized, and subsequently stained with 0.06 μg of FITC-conjugated rat anti-mouse IL-2 antibody (FITC-JES6-5H4, Cat. No. 554427, left panel). To demonstrate specificity of staining, the binding by the FITC-JES6-5H4 antibody was blocked by preincubation of the conjugated antibody with recombinant mouse IL-2 (0.12 μg, Cat. No. 550069, middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled JES6-5H4 antibody (2.5 μg, Cat. No. 554425, right panel) prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls.

BD Pharmingen™ Purified Rat Anti-Mouse IL-2 - Purified - Clone JES6-5H4 - Isotype Rat IgG2b - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse IL-2 Recombinant Protein

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL-2 antibody (ABIN2689752)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation