IL-3 antibody (Biotin)

Catalog No. ABIN2689759

Cited in 4+ publications. The Biotin-conjugated Rat Monoclonal anti-IL-3 antibody (Clone MP2) (ABIN2689759) specifically detects IL-3 in WB and ELISA. The antibody is reactive with Mouse samples.

Quick Overview for IL-3 antibody (Biotin) (ABIN2689759)

Target: IL-3 (Interleukin 3 (IL-3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Clone: MP2

Product Details anti-IL-3 Antibody (Biotin)

Characteristics: The MP2-43D11 antibody reacts with mouse interleukin-3 (IL-3). The immunogen used to generate this hybridoma was recombinant mouse IL-3. This is a non-neutralizing antibody.

BD Pharmingen™ Biotin Rat Anti-Mouse IL-3 - Biotin - Clone MP2-43D11 - Isotype Rat IgG2a - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant mouse IL-3

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IL-3 antibody (Biotin) (ABIN2689759)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Cockayne, Muchamuel, Grimaldi, Muller-Steffner, Randall, Lund, Murray, Schuber, Howard: "Mice deficient for the ecto-nicotinamide adenine dinucleotide glycohydrolase CD38 exhibit altered humoral immune responses." in: Blood, Vol. 92, Issue 4, pp. 1324-33, (1998) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Abrams, Pearce: "Development of rat anti-mouse interleukin 3 monoclonal antibodies which neutralize bioactivity in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 1, pp. 131-7, (1988) (PubMed).

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL-3

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy