IL-4 antibody (Interleukin 4)

Details for Product anti-IL4 Antibody No. ABIN2689762, Supplier: Log in to see
Antigen
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Alternatives
anti-Human IL-4 antibody for Enzyme Immunoassay
Reactivity
Mouse (Murine)
407
228
56
33
25
22
21
16
15
14
13
12
9
8
6
6
6
4
3
2
1
Host
Rat
296
244
216
10
6
4
Clonality (Clone)
Monoclonal ()
Conjugate
This IL-4 antibody is un-conjugated
90
55
41
36
23
13
13
10
9
9
8
7
7
7
7
7
7
5
5
4
4
3
2
2
2
2
1
1
1
1
1
1
Application
Neutralization (Neut), ELISA, Western Blotting (WB)
423
394
279
173
59
54
42
39
38
38
25
22
22
13
8
8
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Brand BD Pharmingen™
Immunogen Recombinant Mouse IL-4
Clone BVD4
Isotype IgG2b
Characteristics The BVD4-1D11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD4-1D11 hybridoma was recombinant mouse IL-4. This is a neutralizing antibody. This antibody is routinely tested by intracellular staining and sandwich ELISA. Other applications were tested during antibody development only or reported in the literature. Expression of IL-4 by MiCK-2 positive control cells. MiCK-2 positive control cells (Cat. No. 554653) were stained with 0.125 μg of FITC-conjugated rat anti-mouse IL-4 antibody by using the BD Pharmingen staining protocol (see left panel). To demonstrate specificity of staining, the binding of FITC-BVD4-1D11 was blocked by the preincubation of the conjugated antibody with recombinant mouse IL-4 (0.25 μg, Cat. No. 550067, see middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled BVD4-1D11 antibody (5 μg, Cat. No. 554386, see right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.

BD Pharmingen™ Purified Rat Anti-Mouse IL-4 - Purified - Clone BVD4-1D11 - Isotype Rat IgG2b - Reactivity Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others IL-4 products on genomics-online (e.g. as negative or positive controls)
Alternative Name IL-4 (IL4 Antibody Abstract)
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Product cited in: Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Litton, Sander, Murphy, OGarra, Abrams: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, 1994 (PubMed).

Finkelman, Madden, Morris, Holmes, Boiani, Katona, Maliszewski: "Anti-cytokine antibodies as carrier proteins. Prolongation of in vivo effects of exogenous cytokines by injection of cytokine-anti-cytokine antibody complexes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1235-44, 1993 (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).

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