IL-6 antibody (Biotin)

Catalog No. ABIN2689780

Cited in 3+ publications. The Biotin-conjugated Rat Monoclonal anti-IL-6 antibody (Clone MQ2) (ABIN2689780) specifically detects IL-6 in WB, ELISA and Neut. The antibody is reactive with Human samples.

Quick Overview for IL-6 antibody (Biotin) (ABIN2689780)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-6 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: MQ2

Product Details anti-IL-6 Antibody (Biotin)

Characteristics: The MQ2-39C3 antibody reacts with human interleukin-6 (IL-6). The immunogen used to generate the MQ2-39C3 hybridoma was recombinant human IL-6. This is a neutralizing antibody. This antibody is routinely tested by ELISA detection. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Human IL-6 - Biotin - Clone MQ2-39C3 - Isotype Rat IgG2a - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant human IL-6

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IL-6 antibody (Biotin) (ABIN2689780)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Gaines Das, Poole: "The international standard for interleukin-6. Evaluation in an international collaborative study." in: Journal of immunological methods, Vol. 160, Issue 2, pp. 147-53, (1993) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome