IL-6 antibody (Biotin)

Catalog No. ABIN2689781

This Rat Monoclonal antibody specifically detects IL-6 in WB and ELISA. It exhibits reactivity toward Mouse. It has been mentioned in 3+ publications

Quick Overview for IL-6 antibody (Biotin) (ABIN2689781)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-6 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Clone: MP5

Product Details anti-IL-6 Antibody (Biotin)

Characteristics: The MP5-32C11 antibody reacts with mouse interleukin-6 (IL-6). The immunogen used to generate the MP5-32C11 hybridoma was recombinant mouse IL-6. This is a non-neutralizing antibody.

BD Pharmingen™ Biotin Rat Anti-Mouse IL-6 - Biotin - Clone MP5-32C11 - Isotype Rat IgG2a - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant mouse IL-6

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IL-6 antibody (Biotin) (ABIN2689781)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Starnes, Pearce, Tewari, Yim, Zou, Abrams: "Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-alpha challenge in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 12, pp. 4185-91, (1991) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome