IL-8 antibody

Catalog No. ABIN2689787

Product Details anti-IL-8 Antibody

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-8 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Blocking Antibody (Inhibition)

Brand: BD Pharmingen™

Characteristics: The G265-8 antibody reacts with both the 72 and 77 amino acid forms of human interleukin-8 (IL-8). The immunogen used to produce the G265-8 hybridoma was E. coli-expressed recombinant human IL-8. Expression of IL-8 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS (10 ng/mL final concentration) in the presence of GolgiStop™ (2 μM final concentration, Cat. No. 554724). The PBMC were harvested, stained with FITC-mouse anti-human CD14 antibody (FITC-M5E2, Cat. No. 555397), fixed, permeabilized, and subsequently stained with 0.25 μg of PE-mouse anti-human IL-8 antibody (PE-G265-8, Cat. No. 554720) following the BD Pharmingen staining protocol (left panel). The data reflect gating on monocytes, based on forward and side scattered light signals. To demonstrate specificity of staining, the binding of PE-G265-8 was blocked by the preincubation of the conjugated antibody with recombinant human IL-8 (0.25 μg, Cat. No. 554609, center panel), and by preincubation of the fixed/permeabilized cells with unlabelled G265-8 antibody (2.5 μg, Cat. No. 554717, right panel) prior to staining with the PE-G265-8 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking (center panel) and unlabelled antibody (right panel) blocking specificity controls.

BD Pharmingen™ Purified Mouse Anti-Human IL-8 - Purified - Clone G265-8 - Isotype Mouse IgG2b - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Human IL-8

Clone: G265

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL-8 antibody (ABIN2689787)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Matsushima, Oppenheim: "Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF." in: Cytokine, Vol. 1, Issue 1, pp. 2-13, (1991) (PubMed).

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: IL-8 (IL8 Products)

Synonyms: CXCL8 antibody, GCP-1 antibody, GCP1 antibody, LECT antibody, LUCT antibody, LYNAP antibody, MDNCF antibody, MONAP antibody, NAF antibody, NAP-1 antibody, NAP1 antibody, IL-8 antibody, AMCF-I antibody, il8 antibody, IL8 antibody, si:dkey-151b16.2 antibody, cxcli2 antibody, cxcl8 antibody, gcp-1 antibody, il-8 antibody, lynap antibody, mdncf antibody, monap antibody, nap-1 antibody, C-X-C motif chemokine ligand 8 antibody, interleukin 8 antibody, chemokine (C-X-C motif) ligand 8a antibody, chemokine (C-X-C motif) ligand 1-like antibody, chemokine (C-X-C motif) ligand 8 L homeolog antibody, CXCL8 antibody, il8 antibody, cxcl8a antibody, IL8 antibody, Cxcl8 antibody, LOC422654 antibody, cxcl8.L antibody

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy