Ly6C antibody (Biotin)

Catalog No. ABIN2689822

Cited in 7+ publications. The Biotin-conjugated Rat Monoclonal anti-Ly6C antibody (Clone AL) (ABIN2689822) specifically detects Ly6C in FACS and IHC (fro). The antibody is reactive with Mouse samples.

Quick Overview for Ly6C antibody (Biotin) (ABIN2689822)

Target: Ly6C (Ly-6C) (Ly-6C)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly6C antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: AL

Product Details anti-Ly6C Antibody (Biotin)

Characteristics: The AL-21 antibody reacts with a non-polymorphic determinant of Ly-6C, a 14- 17- kDa GPI-linked cell-surface antigen found on some monocyte/macrophage populations, granulocytes, endothelial cells, plasma cells, and thymocyte, NK-cell, and T-subsets. Mice with the Ly-6.2 alloantigen (eg, AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129) have subsets of CD8+ and CD4+ Ly-6C+ T cells, while Ly-6.1 strains (eg, A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+ Ly-6C+ T cells. Upregulation of Ly-6C expression on CD8+ T cells by interferons α and β and poly (I:C) has been described, and Ly-6C is a memory marker on CD8+ T cells.

BD Pharmingen™ Biotin Rat Anti-Mouse Ly-6C - Biotin - Clone AL-21 - Isotype Rat IgM, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Not reported

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-Ly6C antibody (Biotin) (ABIN2689822)

Wrammert, Källberg, Agace, Leanderson: "Ly6C expression differentiates plasma cells from other B cell subsets in mice." in: European journal of immunology, Vol. 32, Issue 1, pp. 97-103, (2001) (PubMed).

Cerwenka, Carter, Reome, Swain, Dutton: "In vivo persistence of CD8 polarized T cell subsets producing type 1 or type 2 cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 1, pp. 97-105, (1998) (PubMed).

Sato, Yahata, Santa, Ohta, Ohmi, Habu, Nishimura: "Functional characterization of NK1.1 + Ly-6C+ cells." in: Immunology letters, Vol. 54, Issue 1, pp. 5-9, (1997) (PubMed).

Tough, Borrow, Sprent: "Induction of bystander T cell proliferation by viruses and type I interferon in vivo." in: Science (New York, N.Y.), Vol. 272, Issue 5270, pp. 1947-50, (1996) (PubMed).

Jutila, Kurk, Jutila: "Differences in the expression of Ly-6C on neutrophils and monocytes following PI-PLC hydrolysis and cellular activation." in: Immunology letters, Vol. 41, Issue 1, pp. 49-57, (1994) (PubMed).

Takahama, Sharrow, Singer et al.: "Expression of an unusual T cell receptor (TCR)-V beta repertoire by Ly-6C+ subpopulations of CD4+ and/or CD8+ thymocytes. Evidence for a developmental relationship between Ly-6C+ thymocytes and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 9, pp. 2883-91, (1991) (PubMed).

Jutila, Kroese, Jutila, Stall, Fiering, Herzenberg, Berg, Butcher: "Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma." in: European journal of immunology, Vol. 18, Issue 11, pp. 1819-26, (1989) (PubMed).

Target Details for Ly6C

Target: Ly6C (Ly-6C) (Ly-6C)

Alternative Name: Ly-6C