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PAX6 antibody (AA 406-422)

PAX6 Reactivity: Human WB, IF, BI, ICS Host: Mouse Monoclonal O18 unconjugated
Catalog No. ABIN2689876
  • Target See all PAX6 Antibodies
    PAX6 (Paired Box 6 (PAX6))
    Binding Specificity
    • 27
    • 23
    • 16
    • 15
    • 10
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 406-422
    Reactivity
    • 115
    • 72
    • 68
    • 15
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 96
    • 39
    • 1
    Mouse
    Clonality
    • 86
    • 49
    Monoclonal
    Conjugate
    • 71
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This PAX6 antibody is un-conjugated
    Application
    • 98
    • 50
    • 47
    • 40
    • 30
    • 26
    • 15
    • 14
    • 13
    • 9
    • 8
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)
    Brand
    BD Pharmingen™
    Characteristics
    Pax-6 is a member of the paired box (pax) gene family whose protein products are transcription factors involved in development. Pax family members share a highly conserved DNA binding domain that contains six alpha helices (paired domain)and a homeo box domain. Pax-6 has important roles in the development of the eye, nose, central nervous system, and pancreas. Defects in Pax-6 are responsible for various eye malformations including aniridia and Peters anomaly. The O18-1330 monoclonal antibody reacts with human Pax-6. Because the Pax-6 protein sequence is highly conserved among vertebrate species, cross-reactivity with other species is possible. LEFT: Intracellular staining of Pax-6 in neural induction of human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) were cultured in mTeSR® (Stem Cell Technologies) on plates coated with BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277). Embryoid bodies (EB) were made and cultured in medium containing Knockout™ Serum Replacement (Life Technologies) without bFGF for 24 hours and then in medium containing 250 ng/mL human recombinant noggin (R&D Systems) and 10 mM SB 431542 (Tocris) for 4 more days. The EB were then plated on BD Matrigel-coated plates and grown in medium with ITS supplement (Sigma-Aldrich), noggin, and SB 431542. After growth for 21 days, the cells were collected, fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with either Purified Mouse anti-Human Pax-6 antibody (solid line histrogram) or Purified Mouse IgG2a, k isotype control (dashed line, Cat. No. 554126). The second-step reagent was PE Goat Anti-Mouse Ig (Multiple Adsorption, Cat. No. 550589). Flow cytometry was performed on a BD LSR™ II flow cytometry system. RIGHT: Immunofluorescent staining of Pax-6 in human embryonic stem cell-derived rosettes. H9 human ES cells (WiCell, Madison, WI) passage 54 cultured in mTeSR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were differentiated towards a neural stem cell lineage. Cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1 % Triton™ X-100 [Perm Buffer III is also suitable], and stained with Purified Mouse anti-Human Pax-6 (pseudo-colored green) at 2.5 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies) and counter-staining was with Hoechst 33342 (Sigma-Aldrich, pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. 561462 Rev. 2 Page 1 of 2

    BD Pharmingen™ Purified Mouse anti-Human Pax-6 - Purified - Clone O18-1330 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Pax-6 aa 406-422 Peptide
    Clone
    O18
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product PAX6 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Chambers, Fasano, Papapetrou, Tomishima, Sadelain, Studer: "Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling." in: Nature biotechnology, Vol. 27, Issue 3, pp. 275-80, (2009) (PubMed).

    Osakada, Jin, Hirami, Ikeda, Danjyo, Watanabe, Sasai, Takahashi: "In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction." in: Journal of cell science, Vol. 122, Issue Pt 17, pp. 3169-79, (2009) (PubMed).

    Cerf: "Transcription factors regulating beta-cell function." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 155, Issue 5, pp. 671-9, (2006) (PubMed).

    Glaser, Walton, Maas: "Genomic structure, evolutionary conservation and aniridia mutations in the human PAX6 gene." in: Nature genetics, Vol. 2, Issue 3, pp. 232-9, (1994) (PubMed).

  • Target
    PAX6 (Paired Box 6 (PAX6))
    Alternative Name
    Pax-6 (PAX6 Products)
    Synonyms
    pax6 antibody, an2 antibody, mgda antibody, wagr antibody, pax-6 antibody, pax6a antibody, xpax6 antibody, XLPAX6 antibody, MGC52531 antibody, PAX-6 antibody, AN antibody, AN2 antibody, D11S812E antibody, MGDA antibody, WAGR antibody, Pax-6 antibody, pax6b antibody, 1500038E17Rik antibody, AEY11 antibody, Dey antibody, Gsfaey11 antibody, Sey antibody, PAXZF-A antibody, Pax6.1 antibody, cb280 antibody, fc20e07 antibody, pax-a antibody, pax[zf-a] antibody, paxzfa antibody, wu:fc20e07 antibody, zfpax-6a antibody, zfpax-6b antibody, paired box 6 antibody, paired box 6 L homeolog antibody, paired box 6 S homeolog antibody, paired box 6a antibody, PAX6 antibody, pax6 antibody, Pax6 antibody, pax6.L antibody, pax6.S antibody, pax6a antibody
    Background
    Synonyms: Oculorhombin, Aniridia type II protein, PAX6, AN2 Entrez Gene ID: 5080
    Pathways
    Peptide Hormone Metabolism, Carbohydrate Homeostasis
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