PCNA antibody

Catalog No. ABIN2689877

This Mouse Monoclonal antibody specifically detects PCNA in IHC (f), ICS and BI. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for PCNA antibody (ABIN2689877)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS), BioImaging (BI)

Clone: PC10

Product Details anti-PCNA Antibody

Brand: BD Pharmingen™

Characteristics: The Proliferating Cell Nuclear Antigen (PCNA) was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase δ. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation. Human PCNA has been reported to be 262 amino acids with an apparent molecular weight of 36 kDa. Profile of methanol-fixed MOLT-4 cells analyzed on a BD FACScan™ Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96 well imaging plate (Cat. No. 353219) at ~10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-PCNA antibody. The second step reagent was Alexa Fluor® 555 goat anti-mouse IgG (Invitrogen). The image was taken on a BD Pathway™ 855 Bioimager using a 20x objective. This antibody also stained A549 (ATCC CCL-185) and U-2 OS (ATCC HTB-96) cells. The Triton™ X-100 perm protocol is not recommended for use with this antibody. (See Recommended Assay Procedure).

BD Pharmingen™ Purified Mouse Anti-Human PCNA - Purified - Clone PC10 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Not Reported

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-PCNA antibody (ABIN2689877)

Mathews, Bernstein, Franza, Garrels: "Identity of the proliferating cell nuclear antigen and cyclin." in: Nature, Vol. 309, Issue 5966, pp. 374-6, (1984) (PubMed).

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy