PTCRA antibody

Catalog No. ABIN2689882

Product Details anti-PTCRA Antibody

Target: PTCRA (Pre T-Cell Antigen Receptor alpha (PTCRA))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PTCRA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Characteristics: At very early stages in the intrathymic differentiation of T lymphocytes which will bear αβ T Cell Receptors (TCR), the TCR β chain forms a heterodimer with the pre-TCR α chain (pTα) to form the pre-TCR. This pre-TCR associates with the signal-transducing CD3 complex and controls the survival and proliferation of CD4- CD8- (DN) thymocytes, plays a role in allelic exclusion of the TCR β chain, directly or indirectly regulates TCR δ chain expression, and is eventually replaced by the TCR α chain as thymocytes mature. The 2F5 antibody reacts with the PTα on the surface of CD44- CD25-/lo DN thymocytes.

BD Pharmingen™ Purified Mouse Anti-Mouse Pre-T Cell Receptor α Chain - Purified - Clone 2F5 - Isotype Mouse IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant extracellular (Ig-like) domain of pTalpha

Clone: 2F5

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-PTCRA antibody (ABIN2689882)

Aifantis, Feinberg, Fehling, Di Santo, von Boehmer: "Early T cell receptor beta gene expression is regulated by the pre-T cell receptor-CD3 complex." in: The Journal of experimental medicine, Vol. 190, Issue 1, pp. 141-4, (1999) (PubMed).

Aifantis, Pivniouk, Gärtner, Feinberg, Swat, Alt, von Boehmer, Geha: "Allelic exclusion of the T cell receptor beta locus requires the SH2 domain-containing leukocyte protein (SLP)-76 adaptor protein." in: The Journal of experimental medicine, Vol. 190, Issue 8, pp. 1093-102, (1999) (PubMed).

Wiest, Berger, Carleton: "Control of early thymocyte development by the pre-T cell receptor complex: A receptor without a ligand?" in: Seminars in immunology, Vol. 11, Issue 4, pp. 251-62, (1999) (PubMed).

von Boehmer, Aifantis, Feinberg, Lechner, Saint-Ruf, Walter, Buer, Azogui: "Pleiotropic changes controlled by the pre-T-cell receptor." in: Current opinion in immunology, Vol. 11, Issue 2, pp. 135-42, (1999) (PubMed).

Aifantis, Azogui, Feinberg, Saint-Ruf, Buer, von Boehmer: "On the role of the pre-T cell receptor in alphabeta versus gammadelta T lineage commitment." in: Immunity, Vol. 9, Issue 5, pp. 649-55, (1998) (PubMed).

von Boehmer, Fehling: "Structure and function of the pre-T cell receptor." in: Annual review of immunology, Vol. 15, pp. 433-52, (1997) (PubMed).

Target Details for PTCRA

Target: PTCRA (Pre T-Cell Antigen Receptor alpha (PTCRA))

Alternative Name: Pre-T Cell Receptor alpha Chain (PTCRA Products)

Synonyms: gp33 antibody, pT-alpha antibody, pT-alpha-TCR antibody, pT[a] antibody, pTa antibody, pTalpha antibody, PT-ALPHA antibody, PTA antibody, pre T cell antigen receptor alpha antibody, pre T-cell antigen receptor alpha antibody, Ptcra antibody, PTCRA antibody

Background: Synonyms: pTα

Pathways: Cancer Immune Checkpoints