ROR1 antibody

Catalog No. ABIN2689893

Product Details anti-ROR1 Antibody

Target: ROR1 (Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ROR1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: The 4A5 monoclonal antibody binds to the glycosylated type I membrane protein ROR1 (Receptor-tyrosine-kinase-like Orphan Receptor 1). The ROR1 and ROR2 related proteins are highly conserved evolutionarily and are primarily expressed during embryogenesis. ROR1 is not expressed on normal peripheral blood lymphocytes. ROR1 is similar to tropomyosin receptor kinase (Trk family) neurotropic receptors and shares a cysteine-rich domain with Frizzled receptors for Wnt-family signaling proteins, which are involved in the regulation of embryogenesis and carcinogenesis. Furthermore, there is mounting evidence that aberrant ROR1 expression contributes to human malignancy. ROR1 expression in human embryonal carcinoma cells. Human NCCIT cells (ATCC CRL-2073) were stained with either Purified Mouse IgG2b κ Isotype Control (Cat. No. 555740, dashed line histogram) or Purified Mouse Anti-Human ROR1 (Cat. No. 564464, solid-line histogram) monoclonal antibodies. The second-step reagent was PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable NCCIT cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.

BD Pharmingen™ Purified Mouse Anti-Human ROR1 - Purified - Clone 4A5 - Isotype Mouse IgG2b, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ROR1 cDNA

Clone: 4A5

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ROR1 antibody (ABIN2689893)

Cui, Zhang, Chen, Yu, Widhopf, Fecteau, Rassenti, Kipps: "Targeting ROR1 inhibits epithelial-mesenchymal transition and metastasis." in: Cancer research, Vol. 73, Issue 12, pp. 3649-60, (2013) (PubMed).

Zhang, Chen, Wang-Rodriguez, Zhang, Cui, Frankel, Wu, Kipps: "The onco-embryonic antigen ROR1 is expressed by a variety of human cancers." in: The American journal of pathology, Vol. 181, Issue 6, pp. 1903-10, (2012) (PubMed).

Zhang, Chen, Cui, Chuang, Yu, Wang-Rodriguez, Tang, Chen, Basak, Kipps: "ROR1 is expressed in human breast cancer and associated with enhanced tumor-cell growth." in: PLoS ONE, Vol. 7, Issue 3, pp. e31127, (2012) (PubMed).

Broome, Rassenti, Wang, Meyer, Kipps: "ROR1 is expressed on hematogones (non-neoplastic human B-lymphocyte precursors) and a minority of precursor-B acute lymphoblastic leukemia." in: Leukemia research, Vol. 35, Issue 10, pp. 1390-4, (2011) (PubMed).

Fukuda, Chen, Endo, Tang, Lu, Castro, Widhopf, Rassenti, Cantwell, Prussak, Carson, Kipps: "Antisera induced by infusions of autologous Ad-CD154-leukemia B cells identify ROR1 as an oncofetal antigen and receptor for Wnt5a." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 8, pp. 3047-52, (2008) (PubMed).

Target Details for ROR1

Target: ROR1 (Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1))

Alternative Name: ROR1 (ROR1 Products)

Synonyms: NTRKR1 antibody, dJ537F10.1 antibody, 2810404D04Rik antibody, Ntrkr1 antibody, mRor1 antibody, RGD1559469 antibody, ROR1 antibody, fi34e02 antibody, si:dkey-209n16.1 antibody, wu:fi34e02 antibody, receptor tyrosine kinase like orphan receptor 1 antibody, receptor tyrosine kinase-like orphan receptor 1 antibody, ROR1 antibody, Ror1 antibody, ror1 antibody

Background: Synonyms: Neurotrophic tyrosine kinase, receptor-related 1, NTRKR1

Pathways: RTK Signaling, WNT Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha