SOX1 antibody

Catalog No. ABIN2689905

Product Details anti-SOX1 Antibody

Target: SOX1 (SRY (Sex Determining Region Y)-Box 1 (SOX1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), BioImaging (BI)

Brand: BD Pharmingen™

Characteristics: The N23-844 monoclonal antibody reacts with human Sox1, a member of the SOX [SRY (sex determining region Y)-HMG-box] family of transcription factors. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. It is one of the earliest transcription factors to be expressed in ectodermal cells committed to the neural fate. Sox1 is expressed in both embryonic and somatic neural stem and progenitor cells, and it is down regulated during neuronal differentiation in many neuronal subtypes. Western Blot analysis of SOX1. Lysate from neural stem cells (NSCs) derived from H9 human ES cells (WiCell, Madison, WI) was probed with Purified Mouse anti-Human SOX1 monoclonal antibody at titrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 μg/mL (lane 3). SOX1 is identified as a tight doublet of almost 39 kDa. Immunofluorescent staining of Human SOX1. NSCs derived from H9 human ES cells (WiCell Madison, WI) were fixed, permeabilized with BD Perm/Wash™ Buffer (Cat No. 554723), and stained with purified anti-human SOX1 (0.5 μg/test). The second step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies) and counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. Triton™ X-100 is also suitable for permeabilization.

BD Pharmingen™ Purified Mouse anti-Human SOX1 - Purified - Clone N23-844 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Sox1 Peptide

Clone: N23

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-SOX1 antibody (ABIN2689905)

Kan, Israsena, Zhang, Hu, Zhao, Jalali, Sahni, Kessler: "Sox1 acts through multiple independent pathways to promote neurogenesis." in: Developmental biology, Vol. 269, Issue 2, pp. 580-94, (2004) (PubMed).

Wilson, Koopman: "Matching SOX: partner proteins and co-factors of the SOX family of transcriptional regulators." in: Current opinion in genetics & development, Vol. 12, Issue 4, pp. 441-6, (2002) (PubMed).

Pevny, Sockanathan, Placzek, Lovell-Badge: "A role for SOX1 in neural determination." in: Development (Cambridge, England), Vol. 125, Issue 10, pp. 1967-78, (1998) (PubMed).

Malas, Duthie, Mohri, Lovell-Badge, Episkopou: "Cloning and mapping of the human SOX1: a highly conserved gene expressed in the developing brain." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 8, Issue 11, pp. 866-8, (1998) (PubMed).

Target Details for SOX1

Target: SOX1 (SRY (Sex Determining Region Y)-Box 1 (SOX1))

Alternative Name: SOX1 (SOX1 Products)

Synonyms: BB176347 antibody, Sox-1 antibody, XlSox1 antibody, xSox1 antibody, wu:fi38c08 antibody, wu:fj38h03 antibody, zgc:92865 antibody, SRY-box 1 antibody, SRY (sex determining region Y)-box 1 antibody, SRY-box 1 S homeolog antibody, SRY (sex determining region Y)-box 1b antibody, SRY box 1 antibody, SRY (sex determining region Y)-box 1a antibody, SOX1 antibody, Sox1 antibody, sox1.S antibody, sox1b antibody, sox1a antibody

Molecular Weight: 39 kDa