CD15 antibody

Catalog No. ABIN2689907

This Mouse Monoclonal antibody specifically detects CD15 in FACS, IF, WB, CyTox and BI. It exhibits reactivity toward Human and Mouse and has been mentioned in 5+ publications.

Quick Overview for CD15 antibody (ABIN2689907)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Cytotoxicity Test (CyTox), BioImaging (BI)

Clone: MC480

Product Details anti-CD15 Antibody

Brand: BD Pharmingen™

Characteristics: The MC480 monoclonal antibody reacts with Stage-Specific Embryonic Antigen-1 (SSEA-1), which is a terminal carbohydrate epitope (3-fucosyl-N-acetyllactosamine or 3-FAL) on glycoproteins and lactose series glycolipids. SSEA-1 is related to Lewis blood group antigens and is found in a variety of embryonic and adult tissues and cancers. As its name implies, the expression of SSEA-1 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. However, its expression pattern differs in the human and mouse. In the human, SSEA-1 is not found on embryonic stem (ES) cells, embryonic inner cell mass (ICM), or teratocarcinoma (embryonal carcinoma or EC) cells. As human EC and ES cells undergo differentiation, SSEA-1 expression is upregulated. In the adult, the same epitope is expressed as CD15 on granulocytes and monocytes, but not lymphocytes or dendritic cells. In the mouse, SSEA-1 is found on EC, ES, and primordial germ cells, 8-cell to blastocyst embryos, ICM, and on subpopulations of cells in the adult central nervous system, including stem cells. In contrast to the human, SSEA-1 expression is reduced as mouse EC and ES cells undergo differentiation. Western Blot analysis of SSEA-1 in mouse ES cell line. Lysate from ES-E14TG2a cells (ATCC CRL-1821) was probed with Purified Mouse anti-SSEA-1 monoclonal antibody at titrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 μg/mL (lane 3). High-molecular-weight molecules bearing the SSEA-1 epitope are identified above 200 kDa. Immunofluorescent staining of mouse ES cell line. ES-E14TG2a cells were cultured, fixed, and stained with Purified Mouse anti-SSEA-1 monoclonal antibody (pseudo-colored green) according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor® 647 goat anti-mouse Ig (Invitrogen) and counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer using a 20X objective and merged using BD Attovision™ software. 560079 Rev. 2 Page 1 of 2

BD Pharmingen™ Purified Mouse anti-SSEA-1 - Purified - Clone MC480 - Isotype Mouse IgM, κ - Reactivity Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Teratocarcinoma Cell Line

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD15 antibody (ABIN2689907)

Capela, Temple: "LeX/ssea-1 is expressed by adult mouse CNS stem cells, identifying them as nonependymal." in: Neuron, Vol. 35, Issue 5, pp. 865-75, (2002) (PubMed).

Draper, Pigott, Thomson, Andrews: "Surface antigens of human embryonic stem cells: changes upon differentiation in culture." in: Journal of anatomy, Vol. 200, Issue Pt 3, pp. 249-58, (2002) (PubMed).

Henderson, Draper, Baillie, Fishel, Thomson, Moore, Andrews: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, (2002) (PubMed).

Thomson, Itskovitz-Eldor, Shapiro, Waknitz, Swiergiel, Marshall, Jones: "Embryonic stem cell lines derived from human blastocysts." in: Science (New York, N.Y.), Vol. 282, Issue 5391, pp. 1145-7, (1998) (PubMed).

Solter, Knowles: "Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 75, Issue 11, pp. 5565-9, (1979) (PubMed).

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: SSEA-1

Background: Synonyms: 3-FAL, X-hapten, LeX antigen, CD15

Molecular Weight: multiple, >200 kDa