CD15 antibody

Catalog No. ABIN2689907

Product Details anti-CD15 Antibody

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Cytotoxicity Test (CyTox), BioImaging (BI)

Brand: BD Pharmingen™

Characteristics: The MC480 monoclonal antibody reacts with Stage-Specific Embryonic Antigen-1 (SSEA-1), which is a terminal carbohydrate epitope (3-fucosyl-N-acetyllactosamine or 3-FAL) on glycoproteins and lactose series glycolipids. SSEA-1 is related to Lewis blood group antigens and is found in a variety of embryonic and adult tissues and cancers. As its name implies, the expression of SSEA-1 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. However, its expression pattern differs in the human and mouse. In the human, SSEA-1 is not found on embryonic stem (ES) cells, embryonic inner cell mass (ICM), or teratocarcinoma (embryonal carcinoma or EC) cells. As human EC and ES cells undergo differentiation, SSEA-1 expression is upregulated. In the adult, the same epitope is expressed as CD15 on granulocytes and monocytes, but not lymphocytes or dendritic cells. In the mouse, SSEA-1 is found on EC, ES, and primordial germ cells, 8-cell to blastocyst embryos, ICM, and on subpopulations of cells in the adult central nervous system, including stem cells. In contrast to the human, SSEA-1 expression is reduced as mouse EC and ES cells undergo differentiation. Western Blot analysis of SSEA-1 in mouse ES cell line. Lysate from ES-E14TG2a cells (ATCC CRL-1821) was probed with Purified Mouse anti-SSEA-1 monoclonal antibody at titrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 μg/mL (lane 3). High-molecular-weight molecules bearing the SSEA-1 epitope are identified above 200 kDa. Immunofluorescent staining of mouse ES cell line. ES-E14TG2a cells were cultured, fixed, and stained with Purified Mouse anti-SSEA-1 monoclonal antibody (pseudo-colored green) according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor® 647 goat anti-mouse Ig (Invitrogen) and counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer using a 20X objective and merged using BD Attovision™ software. 560079 Rev. 2 Page 1 of 2

BD Pharmingen™ Purified Mouse anti-SSEA-1 - Purified - Clone MC480 - Isotype Mouse IgM, κ - Reactivity Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Teratocarcinoma Cell Line

Clone: MC480

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD15 antibody (ABIN2689907)

Capela, Temple: "LeX/ssea-1 is expressed by adult mouse CNS stem cells, identifying them as nonependymal." in: Neuron, Vol. 35, Issue 5, pp. 865-75, (2002) (PubMed).

Draper, Pigott, Thomson, Andrews: "Surface antigens of human embryonic stem cells: changes upon differentiation in culture." in: Journal of anatomy, Vol. 200, Issue Pt 3, pp. 249-58, (2002) (PubMed).

Henderson, Draper, Baillie, Fishel, Thomson, Moore, Andrews: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, (2002) (PubMed).

Thomson, Itskovitz-Eldor, Shapiro, Waknitz, Swiergiel, Marshall, Jones: "Embryonic stem cell lines derived from human blastocysts." in: Science (New York, N.Y.), Vol. 282, Issue 5391, pp. 1145-7, (1998) (PubMed).

Solter, Knowles: "Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 75, Issue 11, pp. 5565-9, (1979) (PubMed).

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: SSEA-1 (FUT4 Products)

Synonyms: AI451562 antibody, CD15 antibody, FAL antibody, FucT-IV antibody, LeX antibody, SSEA-1 antibody, Ssea1 antibody, ELFT antibody, FCT3A antibody, FUC-TIV antibody, FUTIV antibody, Fuct antibody, fucosyltransferase 4 antibody, Fut4 antibody, FUT4 antibody

Background: Synonyms: 3-FAL, X-hapten, LeX antigen, CD15

Molecular Weight: multiple, >200 kDa