STING/TMEM173 antibody

Catalog No. ABIN2689910

Product Details anti-STING/TMEM173 Antibody

Target: STING/TMEM173 (TMEM173) (Transmembrane Protein 173 (TMEM173))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This STING/TMEM173 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Intracellular Staining (ICS)

Brand: BD Pharmingen™

Characteristics: The T3-680 monoclonal antibody specifically recognizes human STING (Stimulator of interferon genes protein) which is also known as Endoplasmic reticulum interferon stimulator (ERIS) and Mediator of IRF3 activation (MITA). STING is encoded by TMEM173 (Transmembrane protein 173). STING is a multi-pass transmembrane adaptor protein which is expressed in the endoplasmic reticulum of cells. STING acts as a sensor of cytosolic double-stranded DNA derived from bacteria and viruses and promotes the production of type I interferons, IFN-α and IFN-β. Following activation, STING translocates with TBK1kinase to perinuclear endosomes. The TBK1 kinase phosphorylates and activates interferon regulatory factors, such as IRF3, and NF-κB, which leads to the induction of type I interferon and other immune response genes that play roles in innate and adaptive immunity. Human STING Expression Panel 1. Western blot analysis of STING expression by THP-1 cells. Proteins from human THP-I (Acute monocytic leukemia, ATCC TIB-202) cell lysates were resolved by SDS-PAGE, blotted, and then probed with 0.25 μg/mL (Lane 1), 0.125 μg/mL (Lane 2), and 0.063 μg/mL (Lane 3) of Purified Mouse Anti-Human STING antibody (Cat. No. 564835) followed by HRP-conjugated Goat Anti-Mouse IgG (Cat. No. 554002), and ECL detection reagents. STING was identified as protein bands of ~35-40 kDa. Panel 2. Flow cytometric analysis of STING expression in human peripheral blood leucocytes. Blood was treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leucocytes. Cells were permeabilized and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746, Upper Plot) or Purified Mouse Anti-Human STING (Lower Plot), followed by staining with PE Goat Anti-Mouse Ig (Cat. No. 550589). Contour plots showing the coexpression of STING (or Ig Isotype control staining) versus side light-scatter characteristics (SSC) were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System. Panel 3. Immunohistochemical staining of STING expression in human tonsils. After antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Mouse IgG1 κ Isotype Control (Left Images) or Purified Mouse Anti-Human STING (Right Images). A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was then used. The Anti-STING antibody stained some lymphocytes and endothelial, epithelial, and dendritic cells. Original magnifications: 20X for Upper and 40X for Lower Images. 564835 Rev. 1 Page 1 of 2

BD Pharmingen™ Purified Mouse Anti-Human STING - Purified - Clone T3-680 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human STING Recombinant Protein

Clone: T3

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-STING/TMEM173 antibody (ABIN2689910)

Ablasser, Goldeck, Cavlar, Deimling, Witte, Röhl, Hopfner, Ludwig, Hornung: "cGAS produces a 2'-5'-linked cyclic dinucleotide second messenger that activates STING." in: Nature, Vol. 498, Issue 7454, pp. 380-4, (2013) (PubMed).

Sun, Wu, Du, Chen, Chen: "Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway." in: Science (New York, N.Y.), Vol. 339, Issue 6121, pp. 786-91, (2013) (PubMed).

Zhang, Yuan, Bao, Lu, Kim, Liu: "The helicase DDX41 senses intracellular DNA mediated by the adaptor STING in dendritic cells." in: Nature immunology, Vol. 12, Issue 10, pp. 959-65, (2011) (PubMed).

Unterholzner, Keating, Baran, Horan, Jensen, Sharma, Sirois, Jin, Latz, Xiao, Fitzgerald, Paludan, Bowie: "IFI16 is an innate immune sensor for intracellular DNA." in: Nature immunology, Vol. 11, Issue 11, pp. 997-1004, (2010) (PubMed).

Ishikawa, Barber: "STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling." in: Nature, Vol. 455, Issue 7213, pp. 674-8, (2008) (PubMed).

Target Details for STING/TMEM173

Target: STING/TMEM173 (TMEM173) (Transmembrane Protein 173 (TMEM173))

Alternative Name: STING (TMEM173 Products)

Synonyms: ERIS antibody, MITA antibody, MPYS antibody, NET23 antibody, STING antibody, 2610307O08Rik antibody, Mita antibody, RGD1562552 antibody, transmembrane protein 173 antibody, TMEM173 antibody, Tmem173 antibody

Background: Synonyms: hSTING, MITA, hMITA, ERIS, MPYS, NET23, TM173, TMEM173

Molecular Weight: 35-40 kDa

Pathways: Activation of Innate immune Response