DOT1L antibody (AA 51-150) (HRP)

Catalog No. ABIN2689986

This anti-DOT1L antibody is a Rabbit Polyclonal antibody detecting DOT1L in WB, ELISA, IHC (p) and IHC (fro). Suitable for Human, Mouse, Rat and Green Monkey.

Quick Overview for DOT1L antibody (AA 51-150) (HRP) (ABIN2689986)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reactivity: Human, Mouse, Rat, Green Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOT1L antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DOT1L Antibody (HRP)

Binding Specificity: AA 51-150

Cross-Reactivity: Green Monkey, Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DOT1L

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: DOT1L

Background:

Synonyms: DOT1, KMT4, Histone-lysine N-methyltransferase, H3 lysine-79 specific, DOT1-like protein, Histone H3-K79 methyltransferase, H3-K79-HMTase, Lysine N-methyltransferase 4, DOT1L, KIAA1814

Background: Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

Gene ID: 84444

UniProt: Q8TEK3