DOT1L antibody (AA 51-150) (HRP)

Catalog No. ABIN2689986

The HRP-conjugated Rabbit Polyclonal anti-DOT1L antibody (ABIN2689986) specifically detects DOT1L in WB, ELISA, IHC (p) and IHC (fro). The antibody is reactive with Human, Mouse, Rat and Green Monkey samples.

Quick Overview for DOT1L antibody (AA 51-150) (HRP) (ABIN2689986)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reactivity: Human, Mouse, Rat, Green Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOT1L antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DOT1L Antibody (HRP)

Binding Specificity: AA 51-150

Cross-Reactivity: Green Monkey, Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DOT1L

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: DOT1L

Background:

Synonyms: DOT1, KMT4, Histone-lysine N-methyltransferase, H3 lysine-79 specific, DOT1-like protein, Histone H3-K79 methyltransferase, H3-K79-HMTase, Lysine N-methyltransferase 4, DOT1L, KIAA1814

Background: Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

Gene ID: 84444

UniProt: Q8TEK3