DOT1L antibody (AA 51-150)

Catalog No. ABIN2690105

The Rabbit Polyclonal anti-DOT1L antibody is suitable to detect DOT1L in samples from Human, Mouse, Rat and Green Monkey. It has been validated for WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) and ICC.

Quick Overview for DOT1L antibody (AA 51-150) (ABIN2690105)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reactivity: Human, Mouse, Rat, Green Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOT1L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-DOT1L Antibody

Binding Specificity: AA 51-150

Cross-Reactivity: Green Monkey, Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DOT1L

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: DOT1L

Background:

Synonyms: DOT1, KMT4, Histone-lysine N-methyltransferase, H3 lysine-79 specific, DOT1-like protein, Histone H3-K79 methyltransferase, H3-K79-HMTase, Lysine N-methyltransferase 4, DOT1L, KIAA1814

Background: Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

Gene ID: 84444

UniProt: Q8TEK3