p53 antibody

Catalog No. ABIN2704417

The Mouse Monoclonal anti-p53 antibody has been validated for WB. It is suitable to detect p53 in samples from Human.

Quick Overview for p53 antibody (ABIN2704417)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: GMP Grade

Clone: PAB122

Product Details anti-p53 Antibody

Purpose: Anti-p53 Unconjugated Antibody

Characteristics: p53, a DNA-binding, oligomerization domain- and transcription activation domaincontaining tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes in the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through post-translational modifications, including phosphorylation and acetylation.

Purification: Purified

Purity: >95 %

Immunogen: SV40-transformed, 3T3 clone 4 mouse cells

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS pH 7.2, 0.1 % (w/v) BSA, 0.09 % (w/v) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

NCBI Accession: NM_000546

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect