CTLA4 antibody

Catalog No. ABIN7304772

The Mouse Monoclonal anti-CTLA4 antibody (Clone MDX-01) (ABIN7304772) specifically detects CTLA4 in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for CTLA4 antibody (ABIN7304772)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MDX-01

Product Details anti-CTLA4 Antibody

Purpose: Mouse monoclonal antibody to CD152

Specificity: Recognizes human CD152

Characteristics: Mouse monoclonal antibody to CD152

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD152.

Isotype: IgG2a

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CD152

Background: CD152, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD antigen CD152

Gene ID: 1493

UniProt: P16410

Pathways: Cancer Immune Checkpoints