Integrin beta 3 antibody

Catalog No. ABIN7687678

The Mouse Monoclonal anti-Integrin beta 3 antibody (Clone PM6-13) (ABIN7687678) specifically detects Integrin beta 3 in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for Integrin beta 3 antibody (ABIN7687678)

Target: Integrin beta 3 (ITGB3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PM6-13

Product Details anti-Integrin beta 3 Antibody

Purpose: Mouse monoclonal antibody to CD61

Specificity: Recognizes human CD61

Characteristics: Mouse monoclonal antibody to CD61

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD61.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: CD61

Background: GP3A, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa, CD antigen CD61

Gene ID: 3690

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex