CD8 antibody

Catalog No. ABIN7687689

This Rat Monoclonal antibody specifically detects CD8 in FACS and IF. It exhibits reactivity toward Mouse.

Quick Overview for CD8 antibody (ABIN7687689)

Target: CD8

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 2-43

Product Details anti-CD8 Antibody

Purpose: Rat monoclonal antibody to CD8a

Specificity: Recognizes mouse CD8a

Characteristics: Rat monoclonal antibody to CD8

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified mouse CD8a

Isotype: IgG2b

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD antigen CD8a, CD8B1, T-cell surface glycoprotein CD8 beta chain, CD antigen CD8b

Gene ID: 12525