THRB antibody

Catalog No. ABIN271853

The Rabbit Polyclonal anti-THRB antibody has been validated for WB and IHC (p). It is suitable to detect THRB in samples from Human, Mouse and Rat.

Quick Overview for THRB antibody (ABIN271853)

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THRB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-THRB Antibody

Specificity: This antibody detects endogenous levels of Thyroid Hormone Receptor beta protein. (region surrounding Leu37)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1/5000-1/10000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for THRB

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Alternative Name: THRB / ERBA2

Background: Thyroid hormone nuclear receptors (TRs) are ligand-dependent transcription factors which regulate growth, differentiation and development and represent members of the steroid/retinoic acid superfamily. The two genes encoding TRs identified to date, TRα and TRβ, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements(TREs) with half-site binding motifs in the orientation of palindromes, direct repeats or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3'-triiodo-L-thyronine (T3). Transcriptional regulation by TRs is also modulated by heterodimerization with TR nuclear accessory proteins, the most extensively characterized of which are the retinoid X receptors (RXRα, RXRβ and RXRγ). To a certain extent, this activity is regulated by differential phosphorylation of TRs. Thus, not only are the biological activities of TRs regulated by heterodimerization with RXRs, but in addition, the gene regulatory activities of TRs are linked to other hormonal pathways.Synonyms: NR1A2, Nuclear receptor subfamily 1 group A member 2, THR1, Thyroid hormone receptor beta

Molecular Weight: approx. 42, 55 kDa

Gene ID: 7068

NCBI Accession: NP_000452

UniProt: P10828

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound