CEBPA antibody

Catalog No. ABIN271872

The Rabbit Polyclonal anti-CEBPA antibody (ABIN271872) specifically detects CEBPA in WB and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for CEBPA antibody (ABIN271872)

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CEBPA Antibody

Specificity: This antibody detects endogenous levels of C/EBP-alpha protein.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CEBPA

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Alternative Name: CEBPA

Background: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP alpha, CEBP delta, and CEBP gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL1 response element in the IL6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene.Synonyms: C/EBP alpha, CCAAT/enhancer-binding protein alpha

Molecular Weight: approx. 30.43 kDa

Gene ID: 1050

NCBI Accession: NP_004355

UniProt: P49715

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation