POLR2A/RPB1 antibody

Catalog No. ABIN271906

Product Details anti-POLR2A/RPB1 Antibody

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of POLR2A protein. (region surrounding Pro1613)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A (POLR2A Products)

Synonyms: NCU02103.1 antibody, 19.m02399 antibody, POLR2A antibody, 220kDa antibody, Rpb1 antibody, Rpo2-1 antibody, POLR2 antibody, POLRA antibody, RPB1 antibody, RPBh1 antibody, RPO2 antibody, RPOL2 antibody, RpIILS antibody, hRPB220 antibody, hsRPB1 antibody, DNA-directed RNA polymerase II largest subunit antibody, DNA-directed RNA polymerase II subunit RPB1 antibody, RNA polymerase II large subunit Rpb1 antibody, RNA polymerase II subunit A antibody, DNA-directed RNA polymerase II largest subunit, putative antibody, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antibody, polymerase (RNA) II (DNA directed) polypeptide A antibody, NCU02103 antibody, BBOV_I003360 antibody, EDI_169920 antibody, CMU_000800 antibody, SJAG_01713 antibody, MGYG_01527 antibody, POLR2A antibody, TP03_0185 antibody, EBI_22514 antibody, CGB_E5020W antibody, polr2a antibody, Polr2a antibody

Background: RPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.Synonyms: DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerase II subunit RPB1, POLR2, POLRA, RNA polymerase II, RNA polymerase II 220 kd, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RPB1, RPB220, RPOL2

Molecular Weight: 217,240 kDa

Gene ID: 5430

NCBI Accession: NP_000928

UniProt: P24928

Pathways: Regulatory RNA Pathways