SIX3 antibody

Catalog No. ABIN272150

The Rabbit Polyclonal anti-SIX3 antibody is suitable to detect SIX3 in samples from Human, Mouse and Rat. It has been validated for WB and IF.

Quick Overview for SIX3 antibody (ABIN272150)

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-SIX3 Antibody

Specificity: This antibody detects endogenous levels of Six3/6 protein. (region surrounding Asp228)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for SIX3

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Alternative Name: SIX3

Background: The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Two of these family members Six3 and Six6 (also designated Optx2 and Six9) are required for the specification and proliferation of the eye field in vertebrates, and, therefore, are the vertebrate homologues most closely related to the Drosophila sine oculis protein, which has an essential role in controlling compound eye development. Six3 and Six6 expression largely overlap during development of specific tissues, such as retina, hypothalamus, and pituitary. The human Six6 gene maps to chromosome 14q22.3-q23. Haploinsufficiency of Six6 may cause several developmental disorders, including bilateral anophthalmia and pituitary anomalies. The gene encoding the human Six3 protein maps to chromosome 2p21-p22, a region associated with holoprosencephaly type 2 (HPE2). Deletion of Six3 may be associated with HPE2 disorder, a common, severe malformation of the brain that results from incomplete cleavage of the forebrain during early embryogenesis.Synonyms: Homeobox protein SIX3, Sine oculis homeobox homolog 3

Molecular Weight: approx. 28 kDa

Gene ID: 6496

NCBI Accession: NP_005404

UniProt: O95343

Pathways: Protein targeting to Nucleus