SIX3 antibody (Internal Region)

Catalog No. ABIN6657913

Product Details anti-SIX3 Antibody

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIX3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  This antibody reacts with mouse Six3.   Cross-reactivity with Six3 from other sources has not been determined.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:250

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

ELISA Dilution: 1:10,000-1:15,000

Western Blot Dilution: 1:500 - 1:2,000

IF Microscopy Dilution: 1:200 - 1:1,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Target Details for SIX3

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Alternative Name: Six3 (SIX3 Products)

Synonyms: HPE2 antibody, SIX3 antibody, cb347 antibody, six3.2 antibody, six6 antibody, E130112M24Rik antibody, Six3a antibody, Six3alpha antibody, Six3b antibody, Six3beta antibody, six3 antibody, six3.1 antibody, wu:fc10a08 antibody, SIX homeobox 3 antibody, SIX homeobox 3b antibody, sine oculis-related homeobox 3 antibody, SIX homeobox 3a antibody, sine oculis homeobox homolog 3 antibody, SIX3 antibody, six3b antibody, Six3 antibody, six3a antibody, six3 antibody

Background:

Synonyms: rabbit anti-Six3 antibody, Six-3, Six 3, Homeobox protein SIX3, Sine oculis homeobox homolog 3

Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Gene Name: Six3

Gene ID: 20473, 59939908

UniProt: Q62233

Pathways: Protein targeting to Nucleus