FER antibody

Catalog No. ABIN272171

The Rabbit Polyclonal anti-FER antibody is suitable to detect FER in samples from Human, Mouse and Rat. It has been validated for WB and IF.

Quick Overview for FER antibody (ABIN272171)

Target: FER (FER Tyrosine Kinase (FER))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FER antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-FER Antibody

Specificity: This antibody detects endogenous levels of Fer protein. (region surrounding Asp798)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for FER

Target: FER (FER Tyrosine Kinase (FER))

Alternative Name: FER / TYK3

Background: Fer is a non-receptor protein-tyrosine kinase (nRTK) of the Fes/Fps family, which shares a functional (SH2) domain and is involved in signaling pathways through receptor tyrosine kinases (RTK) and cytokine receptors. The Fes/Fps family is distinct from c-Src, c-Abl and related nRTKs and was originally distinguished as a homolog to retroviral oncoproteins. In vivo, Fer kinaseassembles into homotrimers via conserved coiled-coil domains. The N-terminal coiled-coil domains of Fer can autophosphorylate in trans, thereby regulating their cellular function through differential phosphorylation states. Growth factor exposure can induce tyrosine phosphorylation of Fer and recruitment of Fer to RTK complexes containing p85. Fer is implicated in insulin signaling, cell-cell signaling, human prostatic proliferative diseases, and is involved in the regulation of G1 progression.Synonyms: Proto-oncogene tyrosine-protein kinase FER, Tyrosine kinase 3, c-FER, p94-FER

Molecular Weight: approx. 95 kDa

Gene ID: 2241

NCBI Accession: NP_005237

UniProt: P16591