E2F2 antibody
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- Target See all E2F2 Antibodies
- E2F2 (E2F Transcription Factor 2 (E2F2))
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This E2F2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of E2F-2 protein. (region surrounding Lys236)
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Purity
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product E2F2 Primary Antibody
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- Application Notes
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ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- DO NOT FREEZE!
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- Target
- E2F2 (E2F Transcription Factor 2 (E2F2))
- Alternative Name
- E2F2 (E2F2 Products)
- Synonyms
- E2F-2 antibody, CG1071 antibody, Dmel\\CG1071 antibody, E2F antibody, E2F2 antibody, dE2F-2 antibody, dE2F2 antibody, dE2f2 antibody, de2f2 antibody, e2f2 antibody, 9230110J10 antibody, E130207A07 antibody, fb40b03 antibody, wu:fb40b03 antibody, E2F transcription factor 2 antibody, si:ch211-160f23.5 antibody, E2F2 antibody, E2f2 antibody, si:ch211-160f23.5 antibody
- Background
- The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an active E2F transcriptional regulatory complex.Synonyms: E2F transcription factor 2, E2F-2, Transcription factor E2F2
- Molecular Weight
- approx. 48 kDa
- Gene ID
- 1870
- NCBI Accession
- NP_004082
- UniProt
- Q14209
- Pathways
- Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication
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