E2F2 antibody (Internal Region)

Catalog No. ABIN6257150

This Rabbit Polyclonal antibody specifically detects E2F2 in WB, ICC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for E2F2 antibody (Internal Region) (ABIN6257150)

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-E2F2 Antibody

Binding Specificity: Internal Region

Purpose: E2F-2 Antibody

Specificity: E2F-2 Antibody detects endogenous levels of total E2F-2.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human E2F-2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Alternative Name: E2F-2

Background:

Description: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

Gene: E2F2

Molecular Weight: 48 kDa

Gene ID: 1870

UniProt: Q14209

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication