alpha 1 Adrenergic Receptor antibody

Catalog No. ABIN272286

The Rabbit Polyclonal anti-alpha 1 Adrenergic Receptor antibody (ABIN272286) specifically detects alpha 1 Adrenergic Receptor in WB, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for alpha 1 Adrenergic Receptor antibody (ABIN272286)

Target: alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha 1 Adrenergic Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-alpha 1 Adrenergic Receptor Antibody

Specificity: This antibody detects endogenous levels of AR-alpha1A protein. (region surrounding Gln365)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for alpha 1 Adrenergic Receptor

Target: alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))

Alternative Name: alpha-1A Adrenergic Receptor

Background: Alpha1A-adrenergic receptors (alpha1A-ARs) mediate actions in the sympathetic nervous system through the binding of the catecholamines, epinephrine and norepinephrine. alpha1A-AR couples to Gq/11 and regulates blood pressure due to changes in vascular tone and cardiac output. Alternative splicing of the ADRA1A gene generates four isoforms with distinct C-termini, and the different expression profile of these subtypes produces distinct patterns of activation. alpha1A-AR transcripts are abundant in heart, brain, liver and prostate. alpha1A-AR transcript sizes of 6.0, 4.0, 3.0, and 2.0 kb have been detected in liver. Transcripts of 6.0, 4.0 and 3.0 kb have been detected in heart, and transcripts of 6.0 and 4.0 kb have been detected in prostate.Synonyms: ADRA1A, ADRA1C, Alpha 1A-adrenoceptor, Alpha 1A-adrenoreceptor, Alpha-1C adrenergic receptor, Alpha-adrenergic receptor 1c

Molecular Weight: approx. 51 kDa

Gene ID: 148

NCBI Accession: NP_000671

UniProt: P35348

Pathways: AMPK Signaling