POLR2E antibody

Catalog No. ABIN2729316

The Mouse Monoclonal anti-POLR2E antibody (Clone 3B5) (ABIN2729316) specifically detects POLR2E in WB, IHC, IF and FACS. The antibody is reactive with Human, Rat, Mouse, Monkey and Dog samples.

Quick Overview for POLR2E antibody (ABIN2729316)

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Reactivity: Human, Rat, Mouse, Monkey, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLR2E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 3B5

Product Details anti-POLR2E Antibody

Characteristics: Homo sapiens polymerase (RNA) II (DNA directed) polypeptide E, 25 kDa (POLR2E)

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.

Isotype: IgG2a

Application Details

Application Notes: WB 1:500~2000, IHC 1:150, FLOW 1:100, IF: 1:100

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for POLR2E

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Alternative Name: POLR2E

Background: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Molecular Weight: 24.4 kDa

Gene ID: 5434

NCBI Accession: NM_002695

HGNC: 5434

Pathways: Regulatory RNA Pathways