Cyclin D1 antibody (AA 219-238)

Catalog No. ABIN2738934

The Rabbit Polyclonal anti-Cyclin D1 antibody has been validated for WB. It is suitable to detect Cyclin D1 in samples from Human, Mouse and Rat.

Quick Overview for Cyclin D1 antibody (AA 219-238) (ABIN2738934)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cyclin D1 Antibody

Binding Specificity: AA 219-238

Specificity: Human CCND1 / Cyclin D1

Purification: Immunoaffinity purified

Immunogen: Amino acids 219-238 (SPNNFLSCYRTTHFLSRVIK) were used as the immunogen for this Cyclin D1 antibody. This mouse sequence is identical to the related rat sequence, and different from the related human sequence by three amino acids. Percent identity by BLAST analysis: Mouse, Rat (100%), Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey (85%), Orangutan, Galago (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.5 - 1 μg/mL)

Usage: D-type cyclins are regarded as essential links between cell environment and the core cell cycle machinery. Cyclin D1, the product of the human gene CCND1, is also known as BCL1. Cyclin D1 expression is regulated by the retinoblastoma protein. Expression of Cyclin D1, but not of cyclins A and E, was induced by transfection of the RB gene into RB-deficient tumor cells. The CCND1 gene can function as an oncogene.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS, 2.5 % BSA, 0.025 % sodium azide/thimerosal.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 1 month or -20°C for longer. Avoid freeze/thaw cycles.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1 / Cyclin D1

Background: Name/Gene ID: CCND1

Synonyms: CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene

Gene ID: 595

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling