Prostate Specific Antigen antibody

Catalog No. ABIN2742421

This Mouse Monoclonal antibody specifically detects Prostate Specific Antigen in ELISA, IHC, WB, IHC (p), IF, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for Prostate Specific Antigen antibody (ABIN2742421)

Target: Prostate Specific Antigen (PSA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prostate Specific Antigen antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: A67-B-E3

Product Details anti-Prostate Specific Antigen Antibody

Specificity: Human KLK3 / PSA

Purification: Protein G purified

Immunogen: Prostate Specific Antigen from human sperm plasma.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: This antibody recognizes a single protein of 33-34  kDa, identified as the Prostate specific antigen. This PSA antibody is highly specific and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. Prostate specific antigen is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign hyperplasia. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA Buffer,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. ELISA: Order BSA-free PSA antibody format for coating.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: KLK3 / PSA

Background: Name/Gene ID: KLK3
Subfamily: Serine S1
Family: Protease

Synonyms: KLK3, APS, Gamma-seminoprotein, Kallikrein-3, Kallikrein-related peptidase 3, P-30 antigen, Prostate specific antigen, Prostate-specific antigen, PSA, Semenogelase, KLK2A1, Seminin

Gene ID: 354

Pathways: Complement System