KRT8 antibody

Catalog No. ABIN2742508

This Mouse Monoclonal antibody specifically detects KRT8 in WB, IHC, IF, FACS, ELISA, IHC (p) and IP. It exhibits reactivity toward Human, Rat and Zebrafish (Danio rerio).

Quick Overview for KRT8 antibody (ABIN2742508)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Rat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: H1

Product Details anti-KRT8 Antibody

Specificity: Human KRT8 / CK8 / Cytokeratin 8

Purification: Protein G purified

Immunogen: A cytoskeleton preparation containing cytokeratin 8.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (1 - 2 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. antibody to Cytokeratin 8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against Cytokeratin 8, 18 and 19). Reportedly, Cytokeratin 8 antibody is useful for the differentiation of lobular ([Character 93]ring-like, perinuclear[Character 94]) from ductal ([Character 93]peripheral-predominant[Character 94]) carcinoma of the breast. ELISA: Order BSA-free format for coating.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: KRT8 / CK8 / Cytokeratin 8

Background: Name/Gene ID: KRT8
Family: Keratin

Synonyms: KRT8, CARD2, CK-8, CYK8, Cytokeratin-8, CK8, K2C8, K8, KO, Keratin-8, Type-II keratin Kb8, Cytokeratin 8, Keratin 8

Gene ID: 3856