NGFR antibody (Biotin)

Catalog No. ABIN2743244

The Mouse Monoclonal anti-NGFR antibody has been validated for FACS and ELISA. It is suitable to detect NGFR in samples from Human.

Quick Overview for NGFR antibody (Biotin) (ABIN2743244)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), ELISA

Clone: ANC271-3D7

Product Details anti-NGFR Antibody (Biotin)

Specificity: Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.

Purification: Protein A purified

Immunogen: Recombinant soluble human CD271.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Five x 105 cultured human SW480 cells were harvested by EDTA, washed and pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-CD271/Biotin at a concentration of 10 μg/mL. Cells were then washed twice and incubated with secondary reagent Streptaviden/R-PE. They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD271 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR / CD271

Background: Name/Gene ID: NGFR
Family: TNF Receptor

Synonyms: NGFR, CD271, CD271 antigen, gp80-LNGFR, Nerve growth factor receptor, p75 ICD, p75(NTR), p75NTR, TNFR superfamily, member 16, NGF receptor, TNFRSF16

Gene ID: 4804

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding