Melanoma gp100 antibody

Catalog No. ABIN2743687

This Mouse Monoclonal antibody specifically detects Melanoma gp100 in IHC, IF, FACS, ELISA and IHC (p). It exhibits reactivity toward Human and Horse.

Quick Overview for Melanoma gp100 antibody (ABIN2743687)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NKI-beteb

Product Details anti-Melanoma gp100 Antibody

Specificity: Human PMEL / SILV / gp100

No Cross-Reactivity: Rat (Rattus)

Purification: Protein G purified

Immunogen: Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).

Type of Immunogen: Membrane preparation

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (1 - 2 μg/mL), IHC, IHC-P (1 - 2 μg/mL)

Usage:

By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation. ELISA: Order BSA-free format for coating.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: PMEL / SILV / Gp100

Background: Name/Gene ID: PMEL

Synonyms: PMEL, gp100, Melanocyte protein PMEL, Me20m/me20s, p100, PMEL17, Premelanosome protein, SIL, Silver homolog (mouse), Silver, mouse, homolog of, Silver locus protein homolog, ME20-M, Me20-m/me20-s, ME20M, Melanocyte protein mel 17, Melanocyte protein Pmel 17, Silver (mouse homolog) like, D12S53E, ME20, Melanosomal matrix protein17, SILV

Gene ID: 6490