TYMS antibody

Catalog No. ABIN2745139

This Mouse Monoclonal antibody specifically detects TYMS in IF, WB, FACS, IHC, ELISA, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for TYMS antibody (ABIN2745139)

Target: TYMS (Thymidylate Synthetase (TYMS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TMS715

Product Details anti-TYMS Antibody

Specificity: Human TS / Thymidylate Synthase

Purification: Protein G purified

Immunogen: Recombinant human Thymidylate Synthase.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.25 - 0.5 μg/mL)

Usage: This antibody is specific for a protein of 36  kDa, identified as Thymidylate Synthase (TS). Thymidylate synthase converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. Thymidylate synthase is also a critical target for the fluoropyrimidines, an important group of anti-neoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates thymidylate synthase by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of thymidylate synthase protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas. ELISA: Order BSA-free antibody format for coating.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: TS / Thymidylate Synthase

Background: Name/Gene ID: TYMS

Synonyms: TYMS, HST422, Thymidylate synthetase, TMS, TS, Thymidylate synthase, Tsase

Gene ID: 7298

Pathways: Mitotic G1-G1/S Phases