APAF1 antibody (N-Term)

Catalog No. ABIN2745508

The Rat Monoclonal anti-APAF1 antibody (Clone 2-00E-12) (ABIN2745508) specifically detects APAF1 in WB, IHC, ELISA, ICC and IP. The antibody is reactive with Human samples.

Quick Overview for APAF1 antibody (N-Term) (ABIN2745508)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 2-00E-12

Product Details anti-APAF1 Antibody

Binding Specificity: AA 1-464, N-Term

Specificity: Recognizes the CARD domain of human Apaf-1.

Cross-Reactivity: Human

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Does not cross-react with mouse or rat Apaf-1.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: Apaf-1

Background: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity