APAF1 antibody (N-Term)

Catalog No. ABIN2745510

There are 3+ publications for this product available. The Rat Monoclonal anti-APAF1 antibody is suitable to detect APAF1 in samples from Human and Mouse. It has been validated for WB, ELISA, ICC and IP.

Quick Overview for APAF1 antibody (N-Term) (ABIN2745510)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 18H2

Product Details anti-APAF1 Antibody

Binding Specificity: AA 1-97, N-Term

Purpose: anti-Apaf-1, mAb (18H2)

Specificity: Recognizes the CARD domain of human and mouse Apaf-1. Does not cross-react with rat Apaf-1.

No Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Does not cross-react with rat Apaf-1.

Characteristics: Monoclonal Antibody. Recognizes the CARD domain of human and mouse Apaf-1. Does not cross-react with rat Apaf-1. Isotype: Rat IgG2akappa. Clone: 18H2. Applications: ELISA, ICC, IP, WB. Liquid. In PBS containing 0.02 % sodium azide. Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-APAF1 antibody (ABIN2745510)

Oladzad, Nikkhah, Hosseinkhani: "Optimization of Experimental Variables Influencing Apoptosome Biosensor in HEK293T Cells." in: Sensors (Basel, Switzerland), Vol. 20, Issue 6, (2020) (PubMed).

Tashakor, H-Dehkordi, OConnell, Gomez Ganau, Gozalbes, Eriksson, Hosseinkhani, Fearnhead: "A new split-luciferase complementation assay identifies pentachlorophenol as an inhibitor of apoptosome formation." in: FEBS open bio, Vol. 9, Issue 7, pp. 1194-1203, (2020) (PubMed).

Noori, Hosseini, Nikkhah, Hosseinkhani: "Apoptosome formation upon overexpression of native and truncated Apaf-1 in cell-free and cell-based systems." in: Archives of biochemistry and biophysics, Vol. 642, pp. 46-51, (2019) (PubMed).

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: Apaf-1

Background:

Alternate Names/Synonyms: Apoptosis Protease-activating Factor 1

Product Description: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

UniProt: O88879

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity